Isolation of salmonella spp organisms: culture in specific media, eg tetrathionate broth, selenite broth, brilliant green agar suspect salmonella spp colonies tested by standard biochemical techniques, including h 2 s production, urease activity etc serotype determined by slide agglutination at specialist reference laboratory all. Micro lab 1 study play define aseptic explain the difference between aseptic and sterile list the steps in an aseptic transfer from a slant using a loop 1 flame loop 2 remove culture cap with little finger 3 flame open end of tube (keep tilted) 4 touch sterile loop to organism and withdraw m luteus-yellow s marcesens-red p.
This is a nutrient both containing half the sodium chloride level of lb broth (lab169), this lb broth (hi-salt) please note this product has now been discontinued and will be available for a short period. One of the lab assistants at mountain view college has created this video to demonstrate the correct way to aseptically transfer bacteria from broth to broth, broth to slant, and broth to plate this technique is different from what is usually displayed on youtube. 1 resuspend a lyophilized pellet of the desired organism in an appropriate broth (see manufacturer's product insert) 2 transfer several drops to an appropriate plate and streak for isolation incubate the plate for the appropriate time (24 to 72 hours) in the correct temperature and atmosphere 3.
For a broth to slant transfer, obtain a loopful of broth and place at the base of an agar slant medium lightly draw the loop over the hardened surface in a straight or zig-zag line, from the base of the agar slant to the top for a slant to agar deep transfer, obtain the inoculum from the agar slant.
Start studying lab 1 (1-4, 1-5, 1-6, 2-11) learn vocabulary, terms, and more with flashcards, games, and other study tools difficult b/c you had to make sure not to damage the agar & the agar is easy to damage especially when heating up the loop constantly from broth to broth transfer the turbidity was higher than the slant to broth. Aseptic technique & isolation introduction: slant tubes contain medium solidified with again at an angle for a flat surface to inoculate use aseptic technique to open the broth tube, insert the loop to obtain a sample of the culture and replace the cap using aseptic technique.
Insert the loop into the sterile broth and inoculate it by gently moving the loop back and forth in order to disperse the cells remove the loop from the tube and flame the mouth of the tube again replace the cap on the tube and place it on the rack. 4 sterilize the loop again by dipping it in the sand jar and passing it through the flame inoculate an agar plate from a sample of a culture broth of the sample culture 1 sterilize the loop 2 use aseptic technique to open the broth tube, insert the loop to obtain a sample of the culture and replace the cap using aseptic technique. An experiment on s simegmatis broth to broth loop transfer, serratis marcenr to slant, and m luteus steak plate pages 7 words 1,316 view full essay more essays like this: not sure what i'd do without @kibin - alfredo alvarez, student @ miami university exactly what i needed. 4 streak the sterile slant surface with the loop 5 heat mouth of newly inoculating slant tube and replace cap 6 heat loop before returning to its receptacle.
Selenite broth is an enrichment medium that is used to recover salmonella from feces or the intestinal tract (orcutt, 1980) media are categorized as differential when they allow colonies to be morphologically differentiated based on metabolic characteristics. Lb broth product code: lab169 a nutrient broth primarily used for the growth and maintenance of escherichia coli used as the primary propagation step for donor or recipient cells, when further work is to be performed on lb agar.